Blood mRNA Expression Profiles of Autophagy, Apoptosis, and Hypoxia Markers on Blood Cardioplegia and Custodiol Cardioplegia Groups

Abstract Introduction: Blood cardioplegia (BC) and Custodiol (CC) have been used for a long time in open heart surgery are highly effective solutions. The most controversial issue among these two is whether there any difference between them regarding myocardial damage after ischemia surgery. In this study, autophagy, apoptosis, hypoxia markers were investigated that way we evaluated the differences BC CC patients. Methods: A total of 30 patients included using different cardioplegic Three whole blood samples taken from central vein (preoperatively, immediately postoperatively, one day surgery). Total ribonucleic acid was extracted samples. Quantitative real-time polymerase chain reaction performed, changes gene expression determined by 2-∆∆Ct method relative quantification. Results: group, Beclin level found to be higher statistically significant (P=0.0024). Similarly, cysteine-aspartic protease (caspase) 9 hypoxia-inducible factor 1α messenger (mRNA) increased significantly group. microtubule-associated protein light 3 expressions Caspases 8 Conclusion: As result analysis performed groups, it has shown harms myocardium more than at mRNA related markers.